Device

Part:BBa_J100223:Design

Designed by: Julia Preziosi   Group: Campbell_M_Lab   (2015-07-07)


Caffeine Aptamer with RS3, riboswitch in tCloneRed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 821
    Illegal AgeI site found at 933
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to add the aptamer to a riboswitch so that we could get the selective termination via the stem-loop behavior. We therefore did't know how the total riboswitch would fold or if it would be a successful riboswitch. Aptamer alone = GGAUGUCCAGUCGCUUGCAAUGCCCUUUUAGACCCUGAUGAGGAUCAUCGGACUUUGUCCUGUGGAGUAAGUCGCGAAACGGUGAAAGCCGUAGGUCU


Source

tCloneRed = BBa_J119361 Riboswitch 3 = J100207; Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .

References